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unmethylated 236 human control dna  (Zymo Research)


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    Zymo Research unmethylated 236 human control dna
    Unmethylated 236 Human Control Dna, supplied by Zymo Research, used in various techniques. Bioz Stars score: 94/100, based on 244 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/methylated+control+dna/Human+Methylated+%26+Non-Methylated+(WGA)+DNA+Set+(DNA+w%2F+primers)/pm41985722-118-14-19
    Average 94 stars, based on 244 article reviews
    unmethylated 236 human control dna - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Methylation:

    Article Title: Age-related matrix stiffening epigenetically regulates α-Klotho expression and compromises chondrocyte integrity.
    Article Snippet: .. Fully methylated control DNA (Zymo Research, Irvine, CA) was used as a reference to calculate the percentage methylation of DNA samples. .. Levels of 5 methylcytosine (5mC) in DNA were measured using the 5mC DNA ELISA Kit (Zymo Research, Irvine, CA).

    Article Title: Linking STRs/SNPs and DNA methylation using massively parallel sequencing for potential forensic applications.
    Article Snippet: .. Specific and non-preferential amplification was verified using converted non-methylated and methylated control DNA in defined mixtures (Human Methylated and Nonmethylated DNA Set, Zymo Research and EpiTect PCR Control DNA Set, Qiagen) with 0, 10, 25, 50, 75, 90 and 100% methylated DNA. ..

    Article Title: TRPM7 channels regulate breathing during sleep in obesity by acting peripherally in the carotid bodies
    Article Snippet: .. Fully methylated control DNA (Zymo Research) was used as a reference to calculate the percentage methylation of DNA samples. ..

    Article Title: Linking STRs/SNPs and DNA methylation using massively parallel sequencing for potential forensic applications.
    Article Snippet: .. We performed bisulfite sequencing of a 1:1 mixture of non-methylated and methylated control DNA (Human Methylated and Non-methylated DNA Set, Zymo Research) and display FDSTools output of exemplary targets in Fig. 1b. ..

    Article Title: Linking STRs/SNPs and DNA methylation using massively parallel sequencing for potential forensic applications
    Article Snippet: .. We performed bisulfite sequencing of a 1:1 mixture of non-methylated and methylated control DNA (Human Methylated and Non-methylated DNA Set, Zymo Research) and display FDSTools output of exemplary targets in Fig. b. D16S539 consists of alleles 11 and 14 with the repeat pattern GATA[11] and GATA[14]. ..

    Article Title: Imprinted genes and imprinting control regions show predominant intermediate methylation in adult somatic tissues.
    Article Snippet: .. For ‘unmethylated control DNA’, the DNA from subcutaneous adipose tissue was amplified using the GenomiPhi kit (GE Healthcare, NJ, USA), while for ‘methylated control DNA’ the universal methylated human DNA standard (Zymo Research Orange, CA, USA) was used. .. From each sample, 600 ng of genomic DNA was bisulfite modified using the EZ DNA Methylation kit (Zymo Research Orange) according to the manufacturer’s recommendations for the Illumina Infinium Assay.

    Article Title: Exercise-primed extracellular vesicles improve cell-matrix adhesion and chondrocyte health
    Article Snippet: .. Fully methylated control DNA (Zymo Research, Irvine, CA) was used as a reference to calculate the percentage methylation of DNA samples. .. Statistical analysis All statistical analyses were performed using JMP Pro 14 software (SAS Institute, Cary, NC).

    Control:

    Article Title: Age-related matrix stiffening epigenetically regulates α-Klotho expression and compromises chondrocyte integrity.
    Article Snippet: .. Fully methylated control DNA (Zymo Research, Irvine, CA) was used as a reference to calculate the percentage methylation of DNA samples. .. Levels of 5 methylcytosine (5mC) in DNA were measured using the 5mC DNA ELISA Kit (Zymo Research, Irvine, CA).

    Article Title: Linking STRs/SNPs and DNA methylation using massively parallel sequencing for potential forensic applications.
    Article Snippet: .. Specific and non-preferential amplification was verified using converted non-methylated and methylated control DNA in defined mixtures (Human Methylated and Nonmethylated DNA Set, Zymo Research and EpiTect PCR Control DNA Set, Qiagen) with 0, 10, 25, 50, 75, 90 and 100% methylated DNA. ..

    Article Title: TRPM7 channels regulate breathing during sleep in obesity by acting peripherally in the carotid bodies
    Article Snippet: .. Fully methylated control DNA (Zymo Research) was used as a reference to calculate the percentage methylation of DNA samples. ..

    Article Title: Linking STRs/SNPs and DNA methylation using massively parallel sequencing for potential forensic applications.
    Article Snippet: .. We performed bisulfite sequencing of a 1:1 mixture of non-methylated and methylated control DNA (Human Methylated and Non-methylated DNA Set, Zymo Research) and display FDSTools output of exemplary targets in Fig. 1b. ..

    Article Title: Linking STRs/SNPs and DNA methylation using massively parallel sequencing for potential forensic applications
    Article Snippet: .. We performed bisulfite sequencing of a 1:1 mixture of non-methylated and methylated control DNA (Human Methylated and Non-methylated DNA Set, Zymo Research) and display FDSTools output of exemplary targets in Fig. b. D16S539 consists of alleles 11 and 14 with the repeat pattern GATA[11] and GATA[14]. ..

    Article Title: Imprinted genes and imprinting control regions show predominant intermediate methylation in adult somatic tissues.
    Article Snippet: .. For ‘unmethylated control DNA’, the DNA from subcutaneous adipose tissue was amplified using the GenomiPhi kit (GE Healthcare, NJ, USA), while for ‘methylated control DNA’ the universal methylated human DNA standard (Zymo Research Orange, CA, USA) was used. .. From each sample, 600 ng of genomic DNA was bisulfite modified using the EZ DNA Methylation kit (Zymo Research Orange) according to the manufacturer’s recommendations for the Illumina Infinium Assay.

    Article Title: Exercise-primed extracellular vesicles improve cell-matrix adhesion and chondrocyte health
    Article Snippet: .. Fully methylated control DNA (Zymo Research, Irvine, CA) was used as a reference to calculate the percentage methylation of DNA samples. .. Statistical analysis All statistical analyses were performed using JMP Pro 14 software (SAS Institute, Cary, NC).

    Amplification:

    Article Title: Linking STRs/SNPs and DNA methylation using massively parallel sequencing for potential forensic applications.
    Article Snippet: .. Specific and non-preferential amplification was verified using converted non-methylated and methylated control DNA in defined mixtures (Human Methylated and Nonmethylated DNA Set, Zymo Research and EpiTect PCR Control DNA Set, Qiagen) with 0, 10, 25, 50, 75, 90 and 100% methylated DNA. ..

    Article Title: Imprinted genes and imprinting control regions show predominant intermediate methylation in adult somatic tissues.
    Article Snippet: .. For ‘unmethylated control DNA’, the DNA from subcutaneous adipose tissue was amplified using the GenomiPhi kit (GE Healthcare, NJ, USA), while for ‘methylated control DNA’ the universal methylated human DNA standard (Zymo Research Orange, CA, USA) was used. .. From each sample, 600 ng of genomic DNA was bisulfite modified using the EZ DNA Methylation kit (Zymo Research Orange) according to the manufacturer’s recommendations for the Illumina Infinium Assay.

    Polymerase Chain Reaction:

    Article Title: Linking STRs/SNPs and DNA methylation using massively parallel sequencing for potential forensic applications.
    Article Snippet: .. Specific and non-preferential amplification was verified using converted non-methylated and methylated control DNA in defined mixtures (Human Methylated and Nonmethylated DNA Set, Zymo Research and EpiTect PCR Control DNA Set, Qiagen) with 0, 10, 25, 50, 75, 90 and 100% methylated DNA. ..

    Methylation Sequencing:

    Article Title: Linking STRs/SNPs and DNA methylation using massively parallel sequencing for potential forensic applications.
    Article Snippet: .. We performed bisulfite sequencing of a 1:1 mixture of non-methylated and methylated control DNA (Human Methylated and Non-methylated DNA Set, Zymo Research) and display FDSTools output of exemplary targets in Fig. 1b. ..

    Article Title: Linking STRs/SNPs and DNA methylation using massively parallel sequencing for potential forensic applications
    Article Snippet: .. We performed bisulfite sequencing of a 1:1 mixture of non-methylated and methylated control DNA (Human Methylated and Non-methylated DNA Set, Zymo Research) and display FDSTools output of exemplary targets in Fig. b. D16S539 consists of alleles 11 and 14 with the repeat pattern GATA[11] and GATA[14]. ..



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    Image Search Results


    Identification and Validation of Heart-Specific Methylated CpG Sites in PIH1D1 (A) Workflow illustrating the selection criteria for heart-specific methylated CpG sites (HSMCs). CpG sites were filtered by comparing methylation levels in the left atrium to those in 24 other tissues. Sites with methylation levels below 10% in noncardiac tissues were first selected. CpG sites showing a methylation difference >10% between the left atrium and the average of other tissues were further classified as HSMCs. (B) A total of 33 CpG sites met these criteria and were classified as HSMCs. Heatmap showing the methylation β-values of these 33 HSMCs across 25 different human tissues. (C) Scatter plot showing the β-values of the left atrium (x-axis) compared with the average β-values of 24 other tissues (y-axis) with SD error bars. The orange dot highlights PIH1D1 , representing a CpG site of interest for further investigation. (D) DNA methylation β-values of PIH1D1 (cg02184280) across The Cancer Genome Atlas (TCGA) and The Genomic Data Commons (GDC) data sets, including pan-cancer and breast cancer cohorts. β-values remain below 10% in primary tumors, adjacent normal tissues, and metastatic samples, with higher hypomethylation specificity observed in breast cancer patients. (E) Schematic representation highlighting the CpG site cg02184280 (purple) and the design of methylation-specific PCR (MSP) primers (red) and bisulfite pyrosequencing primers (orange). (F) Gel-based MSP shows amplicons in primary human cardiomyocytes (HCM), induced pluripotent stem cell–derived cardiomyocytes (iPSC-CMs), and AC16 cells but not in GES or breast cancer cell lines. Amplicons were also observed in doxorubicin-treated iPSC-CMs, indicating the specificity of the detected CpG sites to cardiac tissue methylation patterns. In vitro methylated human DNA (IVD) was used as a positive control to validate the specificity and efficiency of the methylation-specific primers. “M” indicates detection of methylated PIH1D1 , while a product in the “U” lane indicates unmethylated PIH1D1 .

    Journal: JACC: Basic to Translational Science

    Article Title: Methylated PIH1D1 as a Heart-Specific Biomarker for Anthracycline-Induced Cardiac Remodeling in Breast Cancer Patients

    doi: 10.1016/j.jacbts.2026.101510

    Figure Lengend Snippet: Identification and Validation of Heart-Specific Methylated CpG Sites in PIH1D1 (A) Workflow illustrating the selection criteria for heart-specific methylated CpG sites (HSMCs). CpG sites were filtered by comparing methylation levels in the left atrium to those in 24 other tissues. Sites with methylation levels below 10% in noncardiac tissues were first selected. CpG sites showing a methylation difference >10% between the left atrium and the average of other tissues were further classified as HSMCs. (B) A total of 33 CpG sites met these criteria and were classified as HSMCs. Heatmap showing the methylation β-values of these 33 HSMCs across 25 different human tissues. (C) Scatter plot showing the β-values of the left atrium (x-axis) compared with the average β-values of 24 other tissues (y-axis) with SD error bars. The orange dot highlights PIH1D1 , representing a CpG site of interest for further investigation. (D) DNA methylation β-values of PIH1D1 (cg02184280) across The Cancer Genome Atlas (TCGA) and The Genomic Data Commons (GDC) data sets, including pan-cancer and breast cancer cohorts. β-values remain below 10% in primary tumors, adjacent normal tissues, and metastatic samples, with higher hypomethylation specificity observed in breast cancer patients. (E) Schematic representation highlighting the CpG site cg02184280 (purple) and the design of methylation-specific PCR (MSP) primers (red) and bisulfite pyrosequencing primers (orange). (F) Gel-based MSP shows amplicons in primary human cardiomyocytes (HCM), induced pluripotent stem cell–derived cardiomyocytes (iPSC-CMs), and AC16 cells but not in GES or breast cancer cell lines. Amplicons were also observed in doxorubicin-treated iPSC-CMs, indicating the specificity of the detected CpG sites to cardiac tissue methylation patterns. In vitro methylated human DNA (IVD) was used as a positive control to validate the specificity and efficiency of the methylation-specific primers. “M” indicates detection of methylated PIH1D1 , while a product in the “U” lane indicates unmethylated PIH1D1 .

    Article Snippet: In vitro methylated human DNA (ZYMO) was used as a positive control for methylation.

    Techniques: Biomarker Discovery, Methylation, Selection, DNA Methylation Assay, Derivative Assay, In Vitro, Positive Control